intein meaning in Chinese

内蛋白

In this article , the advanced structure of hybrid peptide mae is predicted with software . the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2 , and then it is cloned into expression vector plasmid ppic9k . after verified by restriction enzyme analyzing and sequencing , the vector is transferred into the eukaryotic host ( yeast pichia
并以已构建的载体ptyb2 - mae为模板，通过pcr扩增出融合基因mae - imein - cbd ，将其克隆于表达质粒ppic9k中，通过鉴定并测序正确后，电转化真核表达宿主? ?毕赤酵母菌株gs115 ，通过营养缺陷型培养基筛选重组子，再利用g418抗性筛选出整合有多拷贝外源基因的重组子。