hydantoin meaning in Chinese
海因
乙内酰脲
脲基乙酸内酰胺
Examples
- Hydantoinase ( ec 3 . 5 . 2 ) hydrolyzes its substrate 5 ' - monosubstantial hydantoin to enantiomerical n - carbamyl - amino acids and in turn , the resulting product can be chemically or enzymatically converted into the corresponding optically active amino acids
海因酶( hydantoinase ， ec3 . 5 . 2 ) ，是一类催化海因、 5 ’ -单替代海因及其衍生物环酰胺键断裂的酰胺水解酶。 - To improve the production and activity of the enzyme , the hydantoin hydrolase gene ( hyuh ) was cloned into e . coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively . two expression plasmids were thus constructed
用pcr技术从质粒puc18 - 169中扩增出l -乙内酰脲水解酶基因( hyuh )和n -氨甲酰基氨基酸水解酶基因( hyuc ) ，分别在大肠杆菌和毕赤酵母中实现了活性表达。 - Its headquarter is located at xinkailu no . 8 , xuanhua district of zhangjiakou city . its main products include ziyun brand civil explosive products series : powdery ammonium nitrate explosive emulsion explosive industrial fuse ; ziyun brand fine chemical products series : glyoxalic acid 5 - 4 - hydroxyphenyl hydantoin p - hydroxyphenylacetic acid p - hydroxyphenylacetamide allantoin daidzein bufexamac ; aaa brand fine chemical products series : ethylamine methanol trimethylacetic acid tert - valeryl chloride ; great wall brand agricultural chemical fertilizer products series : ammonia urea ammonium bicarbonate ; chenguang brand calium carbide chenguang brand acetylene carbon black xuguang brand dissolved acetylene ; and the others as sulphur ammoina liquor oxygen nitrogen , etc .
公司现有主要产品为，紫云牌民爆产品系列：粉状硝铵炸药乳化炸药工业导火索紫云牌精细化工产品系列：乙醛酸对羟基苯海因对羟基苯乙酸对羟基苯乙酰胺尿囊素大豆甙元丁苯羟酸三a牌精细化工产品系列：乙基胺甲醇特戊酸特戊酰氯长城牌农化产品系列：合成氨尿素碳铵晨光牌电石,乙炔炭黑旭光牌溶解乙炔。 - Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine . hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 . n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids
节杆菌( arthrobacter ) bt801是由军事医学科学院生物工程研究所保存的l -乙内酰脲酶产酶菌株， puc18 - 169是由本室构建的含有节杆菌bt801的l -乙内酰脲酶完整操纵子序列的亚克隆质粒。 - With the procedures , the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein . the purification factor was about 4 . 7 folds with the purity about more than 95 % as estimated by sds - page analysis . d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e . coli strains , respectively
对其中产酶活性最高的一工程菌pexsec一hdt /云coljblz ] ( de3 )海因酶的表达条件进行了研究，目的蛋白的表达量约占总菌蛋白的20 % ，其酶活力为0 . 92u / ml ，约是原始菌株的d一海因酶表观活性的4 . 6倍。