hlf meaning in Chinese
factor]不耐热因素
黄韧带肥厚
Examples
- Fire - fighting vehicles - part 11 : group pumping appliance lf 20 16 , group pumping appliance for rescue operations hlf 20 16
消防车辆.第11部分:地面排水设备lf 20 16救援用排 - The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps . then it is used in the following experiments . the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment
本研究将含有人乳铁蛋白( hlf )基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 ’端调控序列的乳腺特异表达重组载体plncxhlf按常规方法提取、纯化、 pcr 、酶切鉴定后，用于细胞表达和精子介导转基因动物的研究。 - The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein , with mw of 34 kd . the highest amount detected with elisa reached 65mg / l medium / 105 cells . the recombinant hlf protein has the effect of inhibiting e . coli proliferation , whose activity is 1 . 4 - 1 . 8 times higher than the commercially available hlf
诱导后，培养液上清通过western - blotting分析证明，转染细胞表达并分泌出人乳铁蛋白，分子量为34kd ； elisa检测重组蛋白最高表达量为65mg l培养基10 ~ 5细胞；抗菌实验表明，所获得的重组人乳铁蛋白具有抑制大肠杆菌生长的作用，而且比人乳铁蛋白标准品作用更强。