hgm meaning in Chinese
灰质异位症
猪胃粘蛋白
Examples
- Human granulocyte - macrophage colony stimulating factor ( hgm - csf ) is a glycoprotein that stimulates the production of granulocftes , macrophages , and white blood cell
粒细胞巨嗜细胞集落刺激因子( gm - csf )是一种造血生长因子,它参与造血调节过程。 - Expression of hgm - csf in bombyx mori cells and larvae of silkworms has been successful , but it ' s quantity of expression is much less than bmnpv
近年来,在gm - csf基因工程研究方面得到了迅速发展,如在哺乳动物细胞、酵母、大肠杆菌、昆虫细胞中都得到了成功表达。 - In order to enhance it ' s quantity of expression in bombyx mori cells and larvae of silkworms , we co - express bmnpv and hgm - csf in bombyx mori cells and larvae of silkworms , and investigate it ' s outcome
因此,本次实验中,我们将家蚕杆状病毒多角体蛋白与gm - csf融合表达,并研究其对gm - csf表达量的影响。 - Western blotting assay showed that product expression in larvae of silkworm was three proteins , and weights were 17kd , 20kd , and 29kd . because quantity of expression was n ' t enhanced , the co - expression of methology in this study is not desirable
4 、合前后表达产物的eusa测定和生物活性测定比较,融合后产物在表达量增加10 %情况下,生物活性并升高,推测可能与hgm一csf的空间结构有关。 - Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak . 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells . the homologous recombination occurred inside the cells , and the recombinant virus bacpak - 6aa - hgm - csf was expressed , as identified by pcr and southern hybridization . the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
本研究首先通过pcr将家蚕杆状病毒多角体蛋白起始密码子后的18个碱基引入到hgm - csf基因的5 ’端之前,然后将融合基因重组与家蚕杆状病毒转移载体pbacpak8中,获得重组转移载体pbacpak8 - 6aa - hgm - csf ,并与线性化bm - bacpak6dna共转染家蚕细胞株,获得重组病毒bacpak - 6aa - hgm - csf 。