gimsa meaning in Chinese
希姆萨
Examples
- Primary culture of rat preadipocyte were prepared from the epididymal , inguinal and perirenal the fat pads of male normal , healthy , 15 - 20 days sprague - dawley rats . the preadipocyte grew better under the condition of 37 , 95 % humidity , 5 % co2 , ph 7 . 0 - 7 . 2 , centrifuged at 1000r / min , m199medium , and 10 % fetal bo vine serum , seeded at a density of 4 l04 , 5 l04 , / cm2 . oil red o staining was the special method to distinguish adipocyte from other cells , gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop , size and the position of the nucleolus of the staining fat cell
经过多次实验,确定本实验室大鼠前体脂肪细胞的最佳培养条件是:温度为37 ,湿度为95 , co _ 2浓度为5 , ph值为7 . 0 7 . 2 ,离心力为1000r / min ,培养基为m _ ( 199 )培养基,胎牛血清浓度为10 ,合适细胞接种密度为4 10 ~ 4 、 5 10 ~ 4个/ cm ~ 2 ,染色结果表明:油红o染色是鉴定脂肪细胞的特异方法, gimsa和he染色可根据不同区域染色程度、着色差别判断细胞核的位置及脂滴大小、多少,观察大鼠前体脂肪细胞分化过程中的形态变化,进而确定脂肪细胞的分化阶段。