gene-specific meaning in Chinese
基因独特性
Examples
- By race pcr , amplified products were obtained with all the gene - specific primers and the adaptor primers
由氨基酸序列推断的ejo基因编码蛋白的等电点为6 18 ，分子量为28 - Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains , three 618 bp cdna fragments ( 618 - 1 . 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves , and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence
用rt - pcr技术，以cdpks激酶区和连接区的保守氨基酸设计的一对简并引物，从蚕豆叶片中还扩增到了3个618bp ( 618 - 1 、 618 - 2和618 - 3 )的cdna片段；用race技术，以618 - 1序列设计的一对基因特异性引物，克隆到了还缺少5 ’末端的cdna片段vfcpk2 。 - Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。