echistatin meaning in Chinese
锯鳞血抑
Examples
- Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible
结论成功构建了echistatin的原核高效表达克隆，表达量高于现有国内外研究水平，为echistatin功能及相关疾病的研究奠定了基础。 - Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。 - Results : designed and synthesized the coding gene of echistatin . and constructe its single copy expression vector , identify the vector by dna sequencing . but it could not express echistatin in e . coli . designed and synthesized the modified coding dna of echistatin , and constructe its two copy expression vector , identify the vector by dna sequencing , but it could not express echistatin in e . coli
结果设计并合成了echistatin编码基因dna序列，构建了echistatin单拷贝表达载体，经dna测序鉴定正确后， sds - page及western - blotting结果表明： echistatin在上述菌株中未获得高效表达。 - Constructed the fusion protein expression vector of echistatin , . and identify it by dna sequencing . the vector can express echistatin fusion protein at a high level , the fusion protein molecular weight is about 16000 da in sds - page analysis , and the fusion protein consists more than 30 % of total protein , the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody . the fusion protein include : hpk5 . his + 6tag , and echistatin . echistatin can be released by cnbr cleavage
构建了echistatin融合蛋白表达载体，经dna测序鉴定正确后，温控诱导表达，获得了高效表达，表达产物经505一队ge分析，分子量为16000oa ，符合预计的融合蛋白分子量，表达产物. ll ’菌体总蛋白的30 %以上， westem一blotting结果显示，该日的蛋白能够和echistatin多克隆抗体发生特异性抗原抗体反应，表明我们成功构建了eohistatin的高效融合表达克隆。