dna重组技术 meaning in Chinese
recombinant dna technique
Examples
- This work is the analysis of metabolic pathway and designing rational genetic modification to optimize cellular properties by using principle of molecular biology
本研究根据代谢工程原理系统分析了细胞代谢网络，并利用dna重组技术合理设计细胞代谢途径及其遗传修饰，进而完成细胞特性改造。 - However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration
植酸酶主要存在于微生物和植物中，动物体内也有少量存在，但真正具有开发价值的主要是微生物产生的植酸酶，尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌，并已投入商品化生产。 - Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。 - The pil - 6 cdna fragment was inserted into pgex - 1 ? t plasmid to construct the expression plasmid pgpil - 6 , the recombinant plasmid was digested by bamh i and pst i to identify whether the pil - 6 cdna fragment was inserted into the plasmid in correct orientation , the pgpil - 6 was transformed into e . coli dh5 ? competent cells
用dna重组技术将已克隆的猪il ? 6cdna片断插入pgex ? 1 t质粒，构建了猪il - 6基因的原核表达质粒pgpil - 6 ，转化大肠杆菌，以bamhi酶切筛选、 psti酶切鉴定转化子，获得了含740bp插入克隆基因正确的重组子。 - By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature
利用dna重组技术，将其结构蛋白vp2基因亚克隆至原核表达载体pproex - htb ， iptg诱导后成功表达出与预期大小相符的约72ku的融合蛋白，光密度扫描对表达产物进行初步定量，表明表达产物约占菌体总蛋白的14 。