dna模板 meaning in Chinese
dna templete
Examples
- To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根据端粒酶htert基因1573 ? 1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna 。 - A complete round of transcription involves the recruitment of polymerase and general transcription factors to the promoter , rna chain synthesis initiation and polymerase escape form the promoter , rna chain elongation , and finally termination with relaease of polymerase and nascent transcript from the dna template
一个完整的转录循环包括ranp和通用转录因子被招募至启动子、 ran链合成的起始和ranp从启动子的脱逸、 rna链的延伸以及伴随ranp和新生rna链从dna模板释放的转录终止等过程。 - Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment , three methods were used to extract genomic dna of bacillus subtilis , i . e . low melting - point agarose embedding method , sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method . the genomic dna of bacillus subtilis were gained by these methods , and the operated programs of the methods were improved . the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因组dna的提取为了获得用于pcr扩增(长距离pcr扩增和分段pcr扩增)的高纯度、大片段(至少为pcr产物长度的4倍)的dna模板，应用三种方法：低熔点琼脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和细菌基因组dna提取试剂盒法，分别提取获得了枯草杆菌基因组dna ，并对3种方法的操作程序进行了不同程度的改进，结果表明：低熔点琼脂糖包埋法提取的基因组dna片段明显大于后两种方法，采用0 . 5琼脂糖凝胶电泳3h ，仍然跑不出加样孔。