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dctp meaning in Chinese

triphosphate]磷酸脱氧胞苷
磷酸脱氧胞苷,三磷酸脱氧胞苷
三磷酸去氧胞甙
三磷酸脱氧胞苷

Examples

  1. Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna
    采用cartesian基因芯片打印仪,将纯化的cdna片段打印在corning玻片表面,制作了酵母细胞基因表达谱芯片。
  2. Polyclonal anti - cdc2 antibodies , anti - cdc25c monoclonal antibodies and peroxidase - conjugated horse anti goat igg were purchased from sigma chemical co . ( stlouis , mo ) reagents [ a - 32p ] dctp and [ r - 32p ] atp were purchased from yahui biotech - nology co . ltd , china
    在真核细胞中,进入分裂其是依赖于mpf ( mphasepromotingfaetor ) , mpf由edez激酶和eyelinb组成,通过磷酸化在有丝分裂期起作用的底物而发挥作用。
  3. Total cellular rna were extracted , 40 micrograms of the total rna were used in the reverse transcription reaction , using superscript ii reverse transcriptase , oligo ( dt ) i8 primers , and cy3 - dctp or cy5 - dctp for the experimental and control group respectively . the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h
    提取as _ 2o _ 3作用k562细胞前后的总rna ,用superscript逆转录酶逆转录成cdna第一链,并在逆转录的过程中,用cy3 cy5荧光染料分别标记对照组处理组,与自制的k562细胞基因表达谱芯片杂交。
  4. In order to study the expression of 3 - defensins in liver as acute phase response proteins , a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study . the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps . the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
    以小鼠mbd3基因145 ? 169bpcdna序列合成探针,经[ - ~ ( 32 ) p ] atp标记后通过northernblot方法检测mbd3在肝脏中的表达,同时分析了mbd3基因诱导表达的组织特异性,剂效和时效关系;结合mbd3基因启动子区序列分析,以- 164 ? - 179bp双链dna序列合成探针,经[ - ~ ( 32 ) p ] dctp标记后通过电泳迁移率改变实验( emsa )和south ? westernblot方法对参与mbd3在肝脏中诱导表达调节的转录因子进行分析。
  5. Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days . then , we did calculating signal arrays , sequencing , sequence analysis and alignments on genebank etc . finally 229 different ests were got from the cdna library
    用在含有外源激素的培养基上培养5天(花分生组织开始形成)和10天(花分生组织已基本形成)的风信子外植体为材料,构建起cdna文库。利用cdnamicroarrray技术,经过杂交筛选和est分析序列分析,最终从cdna文库中获得了229个不同的外源激素诱导响应的基因序列。

Related Words

  1. dctp deaminase
  2. dctp酶
  3. dctp pyrophosphatase
  4. dctp脱氨酶
  5. dctp焦磷酸酶
  6. dctlc diode capacitor transistor logic
  7. dcto dual coolant temperature override
  8. dctp deaminase
  9. dctp pyrophosphatase
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