dctp meaning in Chinese
triphosphate]磷酸脱氧胞苷
磷酸脱氧胞苷,三磷酸脱氧胞苷
三磷酸去氧胞甙
三磷酸脱氧胞苷
Examples
- Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna
采用cartesian基因芯片打印仪，将纯化的cdna片段打印在corning玻片表面，制作了酵母细胞基因表达谱芯片。 - Polyclonal anti - cdc2 antibodies , anti - cdc25c monoclonal antibodies and peroxidase - conjugated horse anti goat igg were purchased from sigma chemical co . ( stlouis , mo ) reagents [ a - 32p ] dctp and [ r - 32p ] atp were purchased from yahui biotech - nology co . ltd , china
在真核细胞中，进入分裂其是依赖于mpf ( mphasepromotingfaetor ) ， mpf由edez激酶和eyelinb组成，通过磷酸化在有丝分裂期起作用的底物而发挥作用。 - Total cellular rna were extracted , 40 micrograms of the total rna were used in the reverse transcription reaction , using superscript ii reverse transcriptase , oligo ( dt ) i8 primers , and cy3 - dctp or cy5 - dctp for the experimental and control group respectively . the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h
提取as _ 2o _ 3作用k562细胞前后的总rna ，用superscript逆转录酶逆转录成cdna第一链，并在逆转录的过程中，用cy3 cy5荧光染料分别标记对照组处理组，与自制的k562细胞基因表达谱芯片杂交。 - In order to study the expression of 3 - defensins in liver as acute phase response proteins , a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study . the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps . the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探针，经[ - ~ ( 32 ) p ] atp标记后通过northernblot方法检测mbd3在肝脏中的表达，同时分析了mbd3基因诱导表达的组织特异性，剂效和时效关系；结合mbd3基因启动子区序列分析，以- 164 ? - 179bp双链dna序列合成探针，经[ - ~ ( 32 ) p ] dctp标记后通过电泳迁移率改变实验( emsa )和south ? westernblot方法对参与mbd3在肝脏中诱导表达调节的转录因子进行分析。 - Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days . then , we did calculating signal arrays , sequencing , sequence analysis and alignments on genebank etc . finally 229 different ests were got from the cdna library
用在含有外源激素的培养基上培养5天(花分生组织开始形成)和10天(花分生组织已基本形成)的风信子外植体为材料，构建起cdna文库。利用cdnamicroarrray技术，经过杂交筛选和est分析序列分析，最终从cdna文库中获得了229个不同的外源激素诱导响应的基因序列。