cdna第一链 meaning in Chinese
first-strand of cdna
Examples
- Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum , whose isotype was identified to be igm and which was testified to be internal image of gaa
分别以日本血吸虫单克隆抗独特型抗体wbo的杂交瘤细胞株总基因组dna和其提取rna进行rticr合成的cdna第一链为模板，扩增v 。 、 v 。 - Total cellular rna were extracted , 40 micrograms of the total rna were used in the reverse transcription reaction , using superscript ii reverse transcriptase , oligo ( dt ) i8 primers , and cy3 - dctp or cy5 - dctp for the experimental and control group respectively . the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h
提取as _ 2o _ 3作用k562细胞前后的总rna ，用superscript逆转录酶逆转录成cdna第一链，并在逆转录的过程中，用cy3 cy5荧光染料分别标记对照组处理组，与自制的k562细胞基因表达谱芯片杂交。 - The special primers were designed and synthesized after the analysis to the nucleotide sequence homology of etrl recorded in genbank . the total rna extracted from latex was reverse - transcribed into first strain of cdna . the special amplification products ( cdna fragment ) were obtained from pcr when using the first strain of cdna as template
通过对genbank登录的已克隆的植物etr1基因序列进行核苷酸同源性分析，设计特异引物。以胶乳总rna为模板，反转录成cdna第一链，又以cdna第一链为模板进行pcr扩增，得到特异扩增片段。