biosoftwares meaning in Chinese
生物软件
Examples
- Arc1 , thl1 and thl2 , the substrate protein genes of s receptor kinase , were cloned through a series of methods of molecular biology such as pcr , rt - pcr , dna cloning and sequencing . the resultings sequences were highly analysed by using the related biosoftwares on internet , providing new insights in the field of the molecular mechanism of self - incompatibility in plants . the major results are as followings : 1
本文通过pcr和rt - pcr等一系列分子生物学方法克隆了芸薹属植物中的甘蓝和油菜自交不亲和信号传导过程中srk底物蛋白基因arc1 、 thl1和thl2 ，并使用各种相关生物信息学软件对srk底物蛋白基因序列进行了分析，然后在internet网上利用在线软件对蛋白质的结构和功能进行了预测和探讨，以期为芸薹属植物自交不亲和性的分子机理的研究提供新的内容。 - Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method
首先，采用rt - pcr和nest - pcr技术扩增了csfv - jl株和csfv - ln9912株eo基因，插入teasy载体后测序，应用dnastar5 . 0和bioedit软件将其与本实验室已测其它毒株和genebank中登录的csfv代表毒株eo基因序列进行比较，绘制了遗传进化树并预测了eo蛋白的抗原表位、疏水性、等电点等特性。