beta-galactosidase meaning in Chinese
β-半乳糖苷酶
Examples
- Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表达以乳酸克鲁维斯酵母( kluyveromyceslactis )菌株k538的基因组dna为模板，设计引物，利用pcr获得乳糖酶基因klac ，经dna测序验证，得到克隆t1549 。 - No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page , except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ) , which is a selective marker of the wild - type virus . elisa test results suggested the expression of egf in cells , but not in culture supernant . the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
将重组病毒rbmbacph - egf以10moi感染bmn细胞， 72小时后收集培养细胞和上清；培养上清和经超声波处理的细胞样品elisa检测发现胞内样品中存在能与egf抗体免疫反应的产物，粗略估计表达量约6 7 g 2 10 ~ 6个细胞(相当于egf标准品) ；重组病毒rbmbacph - egf穿刺接种5龄家蚕幼虫，每隔24h收集蚕血淋巴，经elisa检测发现第4天表达量最高，根据egf标准曲线计算蚕血淋巴的表达量约32 g ml ； elisa定性实验还发现正常蚕血也存在与egf抗体间交叉反应的物质。