atriplex meaning in Chinese
滨藜属
如沙藜属
Examples
- A genomic dna library of atriplex centralasiatica iljin was constructed using lambda embl3 / bamh i as vector
为了得到badh基因的上游序列，我们以lambdaembl3 budl为载体，构建了盐生植物中亚滨茵的基因组文库。 - A gene encoding choline monooxygenase from atriplex hortansis and the approach to produce plants of salt and drought tolerance . shouyi chen , yiguo shen , jinsong zhang and baoxing du
胆碱单加氧酶基因及培育耐旱耐盐植株的方法.陈受宜,沈义国,张劲松,杜保兴 - We isolated the badh cdna ( acbadh ) from the halophyte atriplex centralasiatica iljin using rt - pcr and the deduced protein sequences showed high similarity with other plants
我们以盐生植物中亚滨藜( atriplexcentralasiaticailjin )为材料，用pt - pcr方法克隆了badhcdna 。 - The library consisted of 1 . 3 x 106 clones with an average insert size of about 18kb . the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin . screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr , we got four positive clones
3x10 ‘个重组噬菌体，插入片段大小约为18kb ，含插入片段的频率为100队以中亚滨蓉甜菜碱醛脱氢酶门adh ）基因近5 ’端的约400hp片段为探针，筛选中亚滨蓉基因组文库，得到了4个阳性克隆。 - Badh cdna ( 1901bp ) included a 66 bp 5 " utr , a 329 bp 3 " utr and a 1506 bp orf encoding a 501 - ammo - acid polypeptide which showed 88 % sequence identity to badh from spinach , sugar beet and atriplex hortensis respectively . the deduced amino acid sequence included a decapeptide sequence " vtlelggksp " , which is highly conserved among general aldehyde dehydrogenases ( aldh ) , and a cysteine residue
Badhcdna全长1901bp ， 5端非编码区66bp ， 3端非编码区329bp ，含有2个可能的加polya信号： aataa ，开放阅读框架1506bp ，编码一个由501个氨基酸构成的多肽，与菠菜、甜菜、山菠菜badh的氨基酸序列同源性均为88 ，其中有醛脱氢酶的保守序列vtlelggksp和半胱氨酸残基。