English translation for "梯度离心"
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- gradient cen trifugation
gradient centrifugation
gradient-centrifugation
Related Translations:
高度梯度: altitudinal gradient 低压梯度: low-pressure gradient 电阻梯度: resistance gradient
- Example Sentences:
| 1. | Purification : density gradient centrifugation ( sucrose & cscl )
分离:密度梯度离心法(蔗糖&氯化铯) | | 2. | Density gradient centrifugation
密度梯度离心 | | 3. | Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats , proliferative activities were compared in the cultued cells from the young , adult and aged rats
方法：应用梯度离心和贴壁培养分离大鼠肺泡型细胞，比较幼年、中年和老年大鼠培养肺泡型细胞的增殖能力。 | | 4. | The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition
采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞，通过免疫细胞化学方法对其进行初步鉴定，以及应用流式细胞仪等对其生长状况进行评估。 | | 5. | In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分试验中，首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液，然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离，通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量，并以伊红y排斥试验测定细胞的存活率。 | | 6. | The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究设计以c tamrna为模板的反义cdna片段，从c ta基因5 ’端第94位到500位核苷酸段设计引物，目的片段407bp ，覆盖第116和188位两个aug密码子，也包含了第一外显子和第二外显子间的剪接位点：用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ；腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒，用氯化铯密度梯度离心法获得纯化的高滴度腺病毒；进行体外基因转移，分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞，观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用，从而达到调控mhc -类分子表达的目的。 | | 7. | Cell morphology was similar no matter what method was used . percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) % . proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method . the maximal cell number was 4 . 67 vs 4 . 10 times that of the initial cell number at cell inoculation
红细胞裂解法较密度梯度离心法细胞贴壁较慢， 36小时才可见零星细胞贴壁，两种方法细胞形态相似。活细胞百分比，红细胞裂解法为（ gi士4 ） ，密度梯度离心治（ 83士5 ） ，有明显差异。传代培养： 2 6代范围内，红细胞裂解法较梯度离心法细胞增殖更旺盛（分别是按种时细胞数的4 | | 8. | When the approach to get a direct binding molecule was proved not fruitful , attention was turned to find binding sources for the purpose of further isolating the binding protein . the first considered and also the relatively easier - obtained materials are some adhesion molecules . results showed , only n - cam 140 showed ambiguous binding
， idin砰proteina磁珠为结合反应载体，从小鼠成年全脑匀浆作为结合起始材料，以蔗糖密度梯度离心法进4行细胞亚组分分离，以sds page显示阳性结合条带，以westernblot确定蛋白身份。 |
- Similar Words：
- "梯度空间" English translation, "梯度孔" English translation, "梯度控制单元" English translation, "梯度冷冻" English translation, "梯度冷凝" English translation, "梯度离心分离法" English translation, "梯度离子交换色谱" English translation, "梯度理查森数" English translation, "梯度理查逊数" English translation, "梯度理论" English translation
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