English translation for "restricted enzyme"
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- 限制性内切酶
Related Translations:
- Example Sentences:
| 1. | The expression vector ppiczaa with the poifn - a gene was constructed by restrict enzyme digestion and ligation , then transformed into e . coli jm109 in order to extract recombinant plasmid from jm109
将此基因与毕赤酵母分泌型表达载体ppicz a连接，构建ppicz a - ifn重组质粒，并用常规cacl _ 2法转化大肠杆菌jm109进行扩增。 | | 2. | A about 1 . 0kb dna fragment was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) . the dna fragment was then successfully cloned into pgem - t - easy vector , which was confirmed by sequence analysis and restrict enzyme identification
将此基因克隆到pgem - t - easy载体中，经pcr和测序鉴定，成功地克隆了a毒株的ha1基因。 | | 3. | It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳属2个种的2个菌株在its区域表现出较高的保守性， 4种限制型内切酶的酶切图谱没有显示出多态性；增加内切酶种类及供试菌株数量，有可能获得具有多态性的限制性内切酶酶切图谱； 9本实验中， its区域的真菌特异性引物与真核生物通用引物对于扩增效果无较大差异，扩增片段长度均为650bp左右； 10根据形态学实验、 eric - pcr实验以及southern杂交实验的结果分析，紫木木耳属种质资源的遗传鉴定和遗传多样性评价耳极有可能是毛木耳种的一个变种; n .本研究中所用的gutc法是一种适用于木耳属菌株基因组洲a快速提取的方法; 12 .传统的形态学分类法和现代的分子生物学分类法，两者的关系是相辅相成，互为验证 | | 4. | Results 1 . restrict enzyme analysis and dna sequencing showed that objective dna segments were correct in sequence and insertion direction . 2 . both expressed fusion proteins were mainly in soluble supernatant . the yield of ea fusion protein is 1 . 6 mg per liter bacteria culture while ed fusion is 2 . 9 mg
经诱导表达超声破碎后， gst ? ed融合蛋白约占表达菌可溶性蛋白的25 ， gst ea融合蛋白约占表达菌可溶性蛋白的18 ； gst ? ea蛋白、 gst ? ed蛋白均主要存在于上清液中，经亲和层析回收融合蛋白，每升诱导菌分别可得gst ? ea蛋白1 |
- Similar Words：
- "restricted endonuclease" English translation, "restricted endorsement" English translation, "restricted entry" English translation, "restricted entry type of surge tank" English translation, "restricted environment" English translation, "restricted exit angle" English translation, "restricted extension line" English translation, "restricted extension station" English translation, "restricted feeding" English translation, "restricted fiber diet" English translation
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