| 1. | Colchicine concentrations were four levels of 100 , 500 , 1000 and 2000 mg / l , and treatment periods were of 1 , 2 , 4 and 6 days for the former and 24 , 48 and 72h for the latter . the results showed that both the two methods can induce polyploid cells , but the former was better than the latter in the induction rate and operating 经染色体数目鉴定表明,这两种方法都能有效的诱导四倍体细胞( 2n = 4x = 48 )的产生,而二倍体染色体数为2n = 2x = 24 ,但是加入培养基法无论在诱导率上还是在操作上都优于液体浸泡法。 |