| 1. | For oversea pili fans who wish to join the free epiil membership , please fill the " oversea epili member application "
海外会员加入霹雳网请利用霹雳网海外会员申请表。 |
| 2. | It also means that the il - 2in the fused pili - il - 2 construct may also have a potential role in stimulating vaccine antigen
利用提取的表达产物进行家兔免疫实验，结果表明该产物具有免疫原性。 |
| 3. | D . nodosus pili is the main protective immunogen against footrot in ruminants . il - 2 is known to be as an adjuvant in many areas
已有很多实验证明il - 2作为佐剂能提高体液免疫和细胞介导的免疫反应。 |
| 4. | We currently do not offer pili store direct online order for the oversea members , for the pili dvd enquiry please check this page
目前霹雳网商品馆并无提供直接的线上订购服务给霹雳网的海外会员，希望订购霹雳剧集珍藏dvd的朋友请前往相关说明页面查询。 |
| 5. | Healthy rabbits were inoculated each with vaccine of the recombinant fused pili - el - 2 protein at 250ul per dose to potentiate the immunogenicity . rabbits inoculated two times at one week interval
扩增此质粒，回收bamh切出的2 . 5kb的基因片段，与pme290 ( bamh酶切后去磷酸化)载体连接。 |
| 6. | Type 1 pili is the important virulence factors on the e . coli in fection in chicken . through the adhering of pili , e . coli adhered on the epidermic cell of aspiratory tract , which was the first step of invading in host
1型菌毛是鸡源致病性大肠杆菌的重要毒力因子，在致病过程中介导细菌吸附于鸡呼吸道粘膜上皮细胞完成入侵的第一步。 |
| 7. | It should meet the following 3 requirements : being set up in line with relevant legal basis , having its full name , organization and location , and capable of taking civil liabilities ; independently possessing and using its assets and assuming its liabilities , and entitled to sign contracts with other institutions ; and making independent accounts of its profits and losses , and capable of com pili ng its own balance sheet
它应同时具备的条件是：依法成立，有自己的名称、组织机构和场所，能够承担民事责任；独立拥有和使用资产，承担负债，有权与其他单位签订合同；独立核算盈亏，能够编制资产负债表。 |
| 8. | The result showed that the homology rate of pila gene among the 5 avian pathogenic e . coli strains tested and one human e . coli were from 89 . 8 % to 91 . 1 % , and the homology rate of amino acid were from 88 . 5 % to 91 . 8 % . the homology rate of pila gene sequence among 5 avian pathogenic e . coli strains tested and avian pathogenic e . coli reported ( serotype o1 , o2 , o78 ) were from 87 . 8 % to 90 . 2 % , and the homology rate of amino acid were from 84 . 6 % to 91 . 2 % . there had homology in avian pathogenic e . coli . there had some common antigen side in type 1 pili of avian pathogenic e . coli
结果表明：运用msha法检测1型菌毛的检出率为80 ( 36 45 ) ， pcr法的检出率为95 . 5 ( 43 45 ) ， pcr方法用于1型菌毛的检测比msha更加敏感、快速、特异性强；选择5株优势血清型鸡源致病性大肠杆菌代表株( o _ ( 89 ) ， o _ ( 119 ) ， o _ ( 141 ) ， o _ ( 127 ) )的1型菌毛pila基因的pcr扩增片段经纯化后，分别定向克隆到puc18质粒的多克隆位点，构建了含有目的基因片段的克隆质粒，并转化到dh5株大肠杆菌载体菌中，筛选获得阳性克隆菌株。 |
| 9. | In this paper , 45 e . coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e . coli by animal test . type 1 pili of 45 strains isolated was detected by msha . the pila gene of 45 avian pathogenic e . coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank . results showed that pcr was more sensitive , faster and more characteristic than msha to detect type 1 pili
本研究将从四川规模化鸡场分离鉴定、经1日龄雏鸡致病性试验得到的鸡源致病性大肠杆菌45株，采用d -甘露糖敏感血凝试验( msha )检测1型菌毛，根据genbank中公布的人源大肠杆菌1型菌毛pila基因序列设计一对引物用pcr扩增鸡源致病性大肠杆菌1型菌毛pila基因。 |
| 10. | In this study , the meaningful results have been achieved , which is the important basic work to research the pili subunit vaccine of avian e . coli , and detect pila gene by nucleric acid probe . moreover , it is significant to research molecular epidemiology , to diagnose , to prevent and treat avian colibacillosis
本研究为鸡源致病性大肠杆菌菌毛基因工程疫苗的研究，制备核酸探针检测pila基因提供了重要材料，对鸡大肠杆菌病的分子流行病学研究、诊断和防治研究具有重要意义。 |
