| 1. | Pcr - sscp analysis on exon 1 of myogenin gene in sheep 侧翼调控区的克隆与序列分析 |
| 2. | The application of pcr - sscp in forensic mtdna typing 分型的法医学应用 |
| 3. | Polymorphism of brucella omp28 analyzed by pcr - sscp 多态性分析 |
| 4. | Pcr - sscp analysis on partial sequence of gonadotropin releasing hormone receptor gene in sheep 3基因外显子2的克隆与序列分析 |
| 5. | Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing 方法利用酶切及sscp技术检测凝固酶基因序列改变情况,并测序进行确证。 |
| 6. | But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation 将表达产物破包涵体后经glutathinonesepharose4b柱亲和层析分离, sds page显示纯化的蛋白质为一分子量60kd的单一蛋白带。 |
| 7. | With strong specificity and easy to manipulation , this simple , inexpensive , rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp , which shows better advantage than pcr - sscp . 2 Mb杂交技术特异性强,简单快捷,能进行可视性检测,完全适用于点突变和snp的研究,技术特点明显优于pcr sscp 。 |
| 8. | The research results further prove that resistance to rifampin in mycobacterium tuberculosis resulted from the rpob gene mutation and pcr - sscp can quickly detect rifampin resistance in mycobacterium tuberculosis clinical isolates 这些研究结果进一步证实,结核分枝杆菌耐利福平是由于rpob基因突变所致,采用pcr - sscp可快速检测结核分枝杆菌对利福平的耐药性。 |
| 9. | Compared with the results of dma direct sequencing , pcr - sscp has the same results as that except for failing to check out the rpob gene mutation of a rifampin - resistant isolate . it proves that pcr - sscp is a rapid and sensitive mutation testing method 与dna测序结果相比较, pcr - sscp除一个耐药株的rpob基因突变没有检出外,其它检测结果均与dna测序结果相一致,这进一步说明pcr - sscp是一种快速、灵敏的突变检测技术。 |
| 10. | To investigate the distribution of gene mutations in lpl and to study the relationship between lpl mutations and plasma lipid , the lpl gene ( exon5 ~ 9 including intron - exon boundaries ) was examined by pcr - sscp analysis , and the amplified products showing abnormal pattern on sscp were sequenced using dideoxy - mediated chain - termination 在外显子5中,检出一杂合子突变,经dna序列测定证实为pro ( 207 ) leu突变杂合子,该患者为高甘油三酯血症病人。 |