| 1. | Charged particle bombardment
带电粒子轰击 |
| 2. | Strawberry calli were transformed by particle bombardment ( pds - 1000 / he gene gun ) with plasmid pby520 containing late embryogenesis abundant protein gene , lea3 , from barley . lea3 gene was regulated by act i and pin ii , bar gene as a selected gene
)的胚胎发生晚期丰富蛋白lea3基因的pby520质粒dna导入草莓愈伤组织细胞( act为启动子， pin为终止子， bar基因为选择基因) 。 |
| 3. | Li lines related to di - center , which can be observed in all sic polytypes after various kinds of particle bombardments or irradiations and can withstand high - temperature annealing up to 1700 ? , were observed with the samples after postannealing above 700 ?
在700温度退火后的样品中观察到d _ i - center ，该中心在幅照或粒子轰击后的sic的各种多型体中都观察到，并能经受高达1700温度的退火。 |
| 4. | The first two reports of soybean transformation used two very different methods to transform soybean : hinchee et al ( 1988 ) used agrobacterium - mediated transformation of cotyledonary nodes while mccabe et al ( 1988 ) used particle bombardment of shoot meristems . soybean transformation reports following these initial papers have been limited and the transformation efficiency of soybean has remained low , the reason is that cells that are regeneration - competent must also be accessible and transformation - competent
目前应用于大豆遗传转化的方法主要有根癌农杆菌介导法，基因枪法，超声波辅助农杆菌法，真空抽滤法，花山东师范大学硕士学位论文粉管导人法等，但其中获得广泛应用的只有根癌农杆菌介导法和基因枪法，但是这两种方法在应用中也有一定的局限性。 |
| 5. | They contained an antisense constructed for the spruce gene encoding ccr ( cinnamoyl alcohol dehydrogenase ) , an enzyme of monolignol synthesis . the antisense rna method is a technique for reducing the expression of a resident target gene . the transgenic sublines were produced by particle bombardment at the dept of forest genetics , swedish university of agricultural sciences
本项目研究的目的就是采用瑞典农业大学构建的反义ccr基因转导的挪威云杉细胞愈伤组织，通过诱导产生再生植株，并检测证实该基因是否表达及其它相关基因的表达状况，为培育出低木质素的转基因挪威云杉新品种奠定物质和理论基础。 |
| 6. | The chimeric gene was transformed into rice calli by particle bombardment . many resistance calli were obtained , and gus activity was detected . in order to confirm the causality of the mutant phenotype and osdd2 gene , we took an approach of functional knockout ofosdd2 gene , with the method rnai ( rna interference )
为了进一步验证本基因的功能，利用获得的包含wrky保守域的cdna片断构建了可能产生rna干涉( rnainterference ， rnai )的载体，利用农杆菌转化法转化水稻，目前已经获得一批转基因苗。 |
| 7. | A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method , female or male gametophytes as the gene recipients , hybridization as the regeneration route and chloramphenicol , hygromycin or basta as selective reagents
本文从转化受体、转化方法、报告基因、再生途径、筛选方法等方面对裙带菜的遗传转化进行了研究。首先，分离并建立了裙带菜雌雄配子体的无性繁殖系，进行了裙带菜的不同再生途径的研究。 |
| 8. | In order to breed maize dwarf mosaic disease ? esistance maize species , the pap gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment , 10 transgenic maize plants were achieved and the pap gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods . the pap gene in one of the transgenic maize plants had been transcription and translated , the ability of the virus resistance is undergoing
为了解决玉米矮花叶病害的防治问题，采用花粉介导法及基因枪法将pap基因分别导入玉米的杂交种及自交系，获得了10株转基因玉米， pcr及southern - blot检测结果表明pap基因已经整合到了玉米基因组中，并且其中有一株pap基因已经得到了表达，病毒抗性鉴定表明转基因玉米能明显推迟玉米矮花叶病害症状的表现。 |
