| 1. | The cotyledons of the mature embryo are either green, yellow or chalky yellow .
成熟胚的子叶是绿色，黄色或浅黄色。 |
| 2. | In vitro culture of mature embryos of ginkgo biloba l
银杏成熟胚的离体培养研究 |
| 3. | The somaclonal variation of regeneration plant derived from mature embryo of perennial ryegrass ( lolium perenne l . were analyzed with rapd makers
摘要以多年生黑麦草成熟胚诱导获得的再生植株为材料，利用rapd标记方法对其体细胞变异进行了研究。 |
| 4. | When mature embryo was used as explant , as for above three lines of wheat , the optimize concentration of 2 , 4 - d for inducting calli and embryonic calli is 4 . 0 mg / l
35 ，而安农92484只能达到16 sl 。在所试的品种中对于成熟胚在2 ， 4d质量浓度为4 |
| 5. | Embryological studies reveal that megaspore and such cells as egg , synergid and antipodals in mature embryo sac initiate the division of forming haploid plants through embryogenesis or callus formation
胚胎学观察揭示大孢子与胚囊内的卵细胞、助细胞和反足细胞均有可能在培养中启动分裂，通过胚状体或愈伤组织形成单倍体植株。 |
| 6. | The embryogenic calli induced from the mature embryos of forty - five genotypes of sweet corn varieties were treated as materials to analyze the relationship between plant regeneration and callus induction rate , callus proliferation and bud points
摘要以45种基因型甜玉米的成熟胚为外植体材料，分析出愈率、愈伤组织增殖量、芽点数与植株再生的关系。 |
| 7. | Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays . this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content . the calli of the transformed sublines a78 - 3 , a78 - 4 , a78 - 5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established
以转基因亚系a78 - 3 、 a78 - 4 、 a78 - 5和未转基因对照a95 : 88 : 22的细胞愈伤组织为实验材料，以dkm和lp - m熟化培养基培养五周后，再以1 4sh萌发培养基培养四周，成功地诱导形成了胚胎，并再生成新的小植株，萌发成活率达到80 。 |
| 8. | But cluster buds can not be obtained from the callus of stems and embryo . it has likely relate to the inner structure of callus . lt is very difficult to get embryoid from mature embryo and its inducing rate is very low , and only 11 . 5 % in the media containing n6 + ba 1 . 5mg / l + naa 0 . 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture
而茎段和胚诱导的愈伤组织基本上没有丛生芽发生，这可能和不同愈伤组织的内部结构有关。成熟胚的胚状体诱导率较低，诱导相当困难，在n6 bai |
| 9. | Mature embryo - derived calli of japonica rice ( oryza sativa l . ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene , neomycin phosphotransferase gene ( nptii ) , driven by a promoter from the ubiquitin gene in maize , a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter , rice actin - gb promoter , near to the right border of t - dna as activation tagging . in this system , co - cultivation was simplified , special selection stages and pre - regeneration stage were omitted , the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced
在一步转化系统中，光照高温条件下培养的水稻愈伤组织从诱导开始经过4周时间就可以达到转化实验的要求，并且简化、优化了整个共培养过程，省去了一筛、二筛和预分化步骤，只用7周的时间就可以初步得到再生转化植株；共191块愈伤组织得到125块抗性愈伤组织，转化频率达到65 . 4 ，最后共得到99棵独立来源的再生植株，抗性愈伤组织再生频率达到79 . 2 。 |
| 10. | Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle , immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens , the transformation rate of the above acceptor was investigated respectively . the results showed that immature embryo after precultured for 4 ~ 6d was the best . in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb , to agrobacterium tumefaciens eha 105 , od value of 0 . 8 was the best
采用农杆菌介导法将bt毒蛋白基因导入水稻同样以上述两种籼稻为主要研究材料，比较了分别以预培养的幼胚和幼穗、幼胚、成熟胚来源的愈伤组织作为转化受体的愈伤组织转化频率，结果表明预培养4 6天的幼胚最适宜作为农杆菌介导转化的受体；其次是来源于幼胚和成熟胚的生长状态良好的胚性愈伤组织。 |
