| 1. | Their numerical results show that they are efficient and robust
杂交法的数值结果显示它们是有效和健壮的。 |
| 2. | Dot blot mccallum mccallum splaques
斑点分子杂交法 |
| 3. | Preliminary study on prediction of radiosensitivity of human tumor cells with fl uorescence in situ hybridization
荧光原位杂交法预测肿瘤放射敏感性的初步研究 |
| 4. | Using the split steps finite element method , the varied - boundary two - dimensional numerical model of diurnal tide and tidal current in the luoyuan bay is constructed
摘要采用不规则三角形网格的分步杂交法，建立罗源湾海域二维变边界全日潮汐和潮流数值模型。 |
| 5. | The analytical methods about foundation - structure interaction have two main categories . they are analytic methods and numerical methods . at present , finite element method , sub - structural analysis method and mixture method are common methods
地基与结构的相互作用的分析方法有解析法和数值解法，目前常用的方法是有限元法和子结构法以及杂交法。 |
| 6. | In this thesis , the application of combined hybrid method to axisymmetric elasticity problem and the optimization of combined hybrid axisymmetric finite elements are discussed in a manner of combination of mathematical analysis with numerical investigation
本文从理论分析和数值实验的角度研究了组合杂交法在轴对称弹性力学问题上的应用和加权系数优化的原则。 |
| 7. | 5 . the mpcr - rflp assay was useful for its reliability in monitoring hbv ymdd mutants . melting curve assay and pcr microplate hybridization - elisa assay should be further improved to increase their sensitivity and specialty
5 . mpcr一rflp法检测ymdd突变株具有较好的可靠性和可行性，是监测拉米夫定耐药株的一种非常有效的方法;熔解曲线法和pcr微板核酸杂交法需要进一步完善以提高敏感性和特异性。 |
| 8. | The modified sequence was cloned into binary vector pbi121 . the constructed expression vector was named pbi121 - bar , then transferred into agrobacterium tumefaciens lba4404 by triparental crossing . thus the project bacterium has been successfully constructed
改造后的目的基因克隆于真核表达载体pbi121 ，构建表达载体pbi121 - bar ，通过三亲杂交法将真核表达载体pbi121 - bar转入农杆菌lba4404 ，成功构建出工程菌。 |
| 9. | In previous study , janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization , and registered in genbank
在前期的研究工作中，本室姜宏等通过建立小鼠隐睾模型，运用抑制消减杂交法( suppressionsubtractivehybridization ， ssh )克隆出24个小鼠睾丸生精细胞凋亡相关基因的est ，并在genbank中登录。 |
| 10. | Methods : 1 ) 12h after irradiation , the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp . 2 ) the content of mda , the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation . 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具体方法为： ( 1 )照射后12h ，收集nih3t3细胞，用流式细胞仪检测各组细胞的细胞周期， pcr - sscp检测抑癌基因p16的变化； ( 2 ) nih3t3细胞照射后立即收集细胞和细胞上清，用试剂盒测量mda含量和sod 、 gsh - px的活性并观察其变化； ( 3 ) western免疫印迹和点杂交法检测照射2h后的各组hela细胞中基质金属蛋白酶- 2 ( mmp - 2 )的表达变化。 |
