高效价 meaning in English
hbig
Examples
- Analysis of high - titer cold agglutinins causing cross - matching test difficult
高效价冷凝集素致交叉配血困难分析 - A case report of discrepancy between forward typing and reverse typing due to high ti - ter cold auto - antibody
高效价自身冷抗体致血型正反定型不符1例 - Antibody is one of the most important reagents commonly used in immunological cell chemistry technology . actually nowadays highly specific and sensitive antibodies are of vital importance to any molecular biological experiment
抗体是免疫细胞化学技术的首要试剂，制备具有高特异性和敏感性的高效价抗体在细胞生物学研究方面具有重要意义。 - In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。 - The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2 , ha order to prepare ndrg2 antibody , the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector . after induced by iptg , the fusion proteins were expressed in e . coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization , rabbits produced high titer antiserum against ndrg2 . then antisemm was absorbed using ndrg2 antigen immobilized on nc filters , the purified product of antiserum shows high special to ndrg2 protein , and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
为制备高效价的ndrgz抗体，分别构建了prset a雌、 pgex4t d唾仓和pgex4tl七三种原核重组表达质粒，并在大肠杆菌中诱导表达出相应的融合蛋白；用全长gstjqdrgz蛋白免疫兔，然后用gst ndrgz人和gstjqdrgze片段加强免疫，经免疫得到了较高效价的兔抗人ndrz多克隆抗血清，利用固定于硝酸纤维素膜上的ndrgz抗原亲和吸附纯化抗血清，提高了ndrgz抗体的特异性；并对包涵体形式表达的6his ndrgz进行初步的分离纯化。