阴离子交换柱 meaning in English
anion exchange column
anion-exchange column
Examples
- In order to remove potentially interfering substances , the sample can be further cleaned on an spe anion exchange cartridge
为了消除潜在的干扰物质，可在一个spe阴离子交换柱上进一步清洗样品。 - 6 - phosphogluconate dehydrogenase ( 6 - pgadase , ec 1 . 1 . 1 . 44 ) was isolated by homogenate , ammunium sulfate fractionation , deae - sepharose chromatography , blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis , and some properties of the enzyme had been studied . a 113 . 8 - fold purification was obtained with a 8 . 2 % yield . the purified enzyme moved as a single electrophoretic band in page
将枯草芽孢杆菌超声波破壁后的粗提取物进行分段盐析、 deae - sepharose阴离子交换柱层析， blue - sepharosecl - 6b特异结合柱层析和sephadexg - 200凝胶过滤等纯化步骤，得到聚丙烯酰胺凝胶电泳为单一蛋白区带，比活为1 . 46u mg的酶制剂。 - The crude extraction of dry pea seed , which was obtained through marination , homogenization , filtration , centrifugation and other methods , was precipitated by 50 mmol / l ( final concentration ) mgq2 . the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger . single eluting peak containing ferritin was obtained finally
2 、豌豆种子铁蛋白的纯化豌豆种子经浸泡、匀浆、过滤、离心等操作得到豌豆铁蛋白粗提液，再用终浓度为50mmol / l的mgcl _ 2盐析等处理后，经aca _ ( 22 )凝胶过滤柱层析和deae -纤维素52 ( de52 )阴离子交换柱层析等方法进行纯化，得到单一的豌豆铁蛋白洗脱峰。 - The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation , deae - sepharose fast flow ion - exchange chromatography , and sephacryl column gel - filtration , and its biochemical and enzymological properties were identified in this study . it was found that the deduced molecular weight of he in sds - page is about 98 . 5 kda , and its proteolytic activity was optimized at ph of 7 . 5 - 8 . 5 and at temperature of 40 , respectively
我们利用67硫酸铵沉淀、 deae - sepharosefastflow阴离子交换柱层析和sephacryl凝胶过滤柱层析，并以酪蛋白为其蛋白酶水解活性的检测底物、以卵膜为其卵壳裂解活性的特异性底物，从卤虫胚胎孵化液中分离纯化出了卤虫的孵化酶分子，其在sds - page电泳中的分子量约为98 . 5kda 。