野生型基因 meaning in English
wild type gene
wildtype gene
Examples
- Wild type gene
野生型基因 - The coding regions of wild bacillus thuringiensis cry1ie and cry1ac gene were modified according to plant preferring codons . the modified crylle and crylac gene were artificially synthesized
本研究根据植物所偏爱密码子原则，改造苏云金芽孢杆菌crylac ， crylie野生型基因的编码区序列，人工合成crylac ， crylie基因。 - To prepare the wild type mbl in prokaryotic system , a pair of primers was designed and synthesized , and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna . a recombinant prokaryotic expression vector , pet28 - mbl , was constructed by inserted the mbl gene into plasmid pet28 ( b ) , and after transfected it into ecoli bl21 ( de3 ) and induced with iptg , recombinant mbl protein was expressed successfully
本实验另选用了原核表达质粒pet28 ( b ) ，根据已构建好的含有mbl野生型基因的t载体pgem - mbl ，设计一对引物， pcr扩增mbl基因，凝胶回收，双酶切pcr产物和pet28 ( b )质粒， t4连接酶连接，转化大肠杆菌dhsa ，氨芋选择培养挑取克隆鉴定。 - Accordingly , ers2 - 1 is still able to confer ethylene insensitivity via a single receptor gene ers1 in the quadruple mutant , but at a highly reduced level compared to its function in the triple mutants . the major difference between the quadruple and triple mutants is the absence of a wild - type ers2 gene in the quadruple mutant , and we propose that the dominance conferred by ers2 - 1 can be mediated and amplified via the wild - type ers2 to the subfamily i receptor ers1
三突变体的遗传背景与四突变体相比，只是在三突变体中保留了ers2的野生型基因，而当这个野生型的ers2基因突变后，对乙烯不敏感的ers2 - 1基因的功能便减弱了，说明显性基因ers2 - 1在etr1 - 7 ； err2 - 3 ； ein4 - 4三突变体中的功能可以经由活化ers2后再传给ers1 ，而不仅仅是直接传给ers1 。