重组酶 meaning in English
recombinant enzymes
recombinase
Examples
- By using a vestigial - ga14 to deliver the tissue specific flipase to the wing , a f1 genetic screen was performed . a number of mutant alleles of known or unknown genes were isolated from this fl screen
通过运用vestigial - gal4 ， uas - flp系统把重组酶专一性地传递到翅中的新遗传筛选体系来进行遗传筛选，由于在f1即进行筛选，故称f1遗传筛选。 - Its cell wet weight increases in 72h and then keeps constant . studies on the partial enzyme characters of recombinant phytase showed that the recombinant phytase had two ph optima ( 2 . 5 - 3 . 0 and 5 . 0 - 5 . 5 ) and a temperature optimum 55 , and with higher activity under the ph ranged from 4 . 5 to 6 . 0
重组植酸酶部分酶学性质的研究结果表明:重组酶在ph值2 . 5一3 . 0和5 . 0一5 . 5时酶活性最高，且在ph4 . 5一6 . 5之间均有较高的酶活性，最适作用温度为55 。 - After the examination of pcr , one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product , a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut
经pcr检测后，有一个不发光细胞克隆扩增出了与对照质粒ploxifn相同的约1000bp的条带，表明发生了替换反应；另一个不发光细胞克隆认为是在cre重组酶的作用下其内部自行发生了剪切作用，剪切了gfp基因，从而只扩增出了载体序列和一个lox位点的约500bp的条带。