重组噬菌体 meaning in English
recombinant phage
Examples
- Application of quantum dots labeling to the interaction between reconstructed - phage display peptide and insulin receptor
量子点荧光标记在重组噬菌体表面展示肽与胰岛素受体相互作用中的应用 - The phagemid particles displaying functional scfvs were rescued by reinfection of helper phage m13ko7 , thus a murine antibody library was obtained
经辅助噬菌体m13ko7超感染回收全部重组噬菌体，此即噬菌体抗体库。 - The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e . coli bl21 . furthermore , library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周龄balb c小鼠，从其脾脏组织分离总rna ，应用重组噬菌体抗体库技术，构建了一个针对牛精子的噬菌体抗体文库。 - The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 - The library consisted of 1 . 3 x 106 clones with an average insert size of about 18kb . the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin . screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr , we got four positive clones
3x10 ‘个重组噬菌体，插入片段大小约为18kb ，含插入片段的频率为100队以中亚滨蓉甜菜碱醛脱氢酶门adh ）基因近5 ’端的约400hp片段为探针，筛选中亚滨蓉基因组文库，得到了4个阳性克隆。