选择性培养基 meaning in English
selective culture medium
selective medium
Examples
- The formulation and application of a selective medi um for identification of pasteurella pneumotropica
嗜肺巴斯德杆菌选择性培养基研制及应用 - Method drip inoculation , aerobic or anaerobic culture in selective medium were used to test each section of gastrointestinal flora qualitatively and quantitatively
方法采用滴注接种法,在选择性培养基上需氧或厌氧培养后定性定量检测胃肠各段菌群。 - A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接;用连接产物转化大肠杆菌jml09感受态细胞,置含amp 、 x - gal和iptg的lb平板上培养12 20小时;挑取白色菌落于选择性培养基扩大培养,碱裂解法小量提取质粒dna ,并进行酶切分析鉴定,结果获得整合有prvge基因的重组质粒pugedna ,并与其它prv分离株进行ge基因序列同源性分析。