转染子 meaning in English
transfectant
transpeptidase
Examples
- The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鉴定、纯化重组质粒后,脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中, g418选择转染子并克隆化培养,经rt - pcr和分子灯塔探针杂交鉴定其mrna转录,获得4株稳定表达54位密码突变型mbl的cho细胞。