转基因种子 meaning in English
genetically modified seed
Examples
- The resulting plasmid , named prok - sod2 , was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation . the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter . transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ) , several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本实验将sod2基因构建到植物表达载体prok中，导入农杆菌后，进行植物遗传转化，实现其在拟南芥中过量表达，在含30mg l的卡那霉素的培养基上筛选获得纯合转基因株系，自交一代获得足够的纯和转基因种子后，对其进行了分子生物学的验证及生理指标的检验。 - Then , we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens . after verified by antibiotic resistance , reporter gene examination , southern blot detection , and genetic segregation analysis , we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene , respectively . further , we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants
对得到的再生烟草植株分别进行了报告基因表达水平检测、 southern - blot鉴定以及t _ 0代转基因种子遗传分离规律分析，分别得到了单拷贝的稳定表达番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的转基因烟草3株、 7株和1株，同时还得到单拷贝的只转化gusa基因的阴性对照转基因烟草3株。