超声破碎 meaning in English
ultrasonication
Examples
- The distribution of the recombinant proteins was analyzed on 12 % sds - page
表达出的蛋白经超声破碎后，取其上清。 - Ultrasonic cell disruptor
超声破碎仪 - Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised , and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst
Ptg诱导表达，超声破碎细胞后，采用亲和层析方法纯化融合蛋白gst十nadc3 ，并以此为抗原免疫新西兰株白兔制备融合蛋白抗体。应用亲和层析的方法对gst十nadc3融合蛋白抗体进行纯化，以去除抗gst抗体。 - Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation . 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies . 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs , prepare a 50 % slurry for batch purification method , pack column with matrix slurry
三、 gst一hbrp重组蛋白的纯化1 .超声破碎细胞，离心，上清和沉淀进行sds一page电泳分析2 .样品处理提取包涵体，变性后，加人用pbs平衡过的以utal肠onesepb抓脱4b ，室温下孵育3 .以utadtionesepharose4b柱纯化以utad云onesepharose4b柱的准备根据毛山lel ，决定纯化所需的gll卫ta1如oneseph娜se4b的柱床体积，用预冷的1xpbs清洗cldtathionese户， se4b ，得到50 %的基质 - Results 1 . restrict enzyme analysis and dna sequencing showed that objective dna segments were correct in sequence and insertion direction . 2 . both expressed fusion proteins were mainly in soluble supernatant . the yield of ea fusion protein is 1 . 6 mg per liter bacteria culture while ed fusion is 2 . 9 mg
经诱导表达超声破碎后， gst ? ed融合蛋白约占表达菌可溶性蛋白的25 ， gst ea融合蛋白约占表达菌可溶性蛋白的18 ； gst ? ea蛋白、 gst ? ed蛋白均主要存在于上清液中，经亲和层析回收融合蛋白，每升诱导菌分别可得gst ? ea蛋白1