诸葛菜 meaning in English
orychophragmus violaceus
Examples
- In vitro culture and plant regeneration of orychophragmus diffusus
铺散诸葛菜的组织培养及植株再生 - Two partially overlapping cdna fragments which were cloned from o . violaceus using the rt - pcr , 3 ' race ( rapid amplification of cdna ends ) and 5 ' race technique was assembled a 1758bp full cdna sequence of epsp synthase ( accession number in genbank : af440389 )
采用rt pcr技术与3 ’ race和5 ’ race的结合，以诸葛菜的总rna为反转录的模板，克隆、拼结出了诸葛菜的epsp合成酶的全长。 dna序列（该段cdan的genbank的登录号为： af440389 ） 。 - The analysis of these two sequences and reported sequences ( three families , 15 sequences in total ) by nj method and the simplest principle shows , the evolutionary trees established by these two methods are consistent and the sequences of the same family constitute one group . the sequences of thaliana and the sequences of brassicaceae each makes up a sub - group in it . the sequences of violaceus distribute in brassicaceae sub - group and this phenomena shows that the relationship is very near
我们对这些序列与已发表的相应序列(合计3个科， 15个序列)用nj法和最简约性分析，结果表明：由两种方法构建的进化树是一致的，同一科的序列形成一组:在十字花科组的分之图中，来自于拟南芥属的序列与来自于芸墓属的序列又各自形成亚组;来自于诸葛菜属的序列分布在芸墓属的分之图中，揭示出诸葛菜属与芸矍属有着极其近的亲源关系。 - A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments . two dna fragments were first cloned from o . violaceus by performing prc which used o . violaceus genome as the template . one has 798 nucleotides and the other 1157 nucleotides , but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
根据同源比较其它高等植物中epsp合成酶基因，找出该基因的保守序列并设计一对寡核苷酸引物，以诸葛菜的总dna为模板进行pcr反应，克隆出了两个epsps基因的片段，其中一条长为797bp ，另一条1157bp ，它们在genbank的登录号为： af440390 、 af440391 。