识别位点 meaning in English
recognition site
Examples
- A further family of fibre optic chemical sensors has either surface atached molecular recognition sites or a reaction chamber for achieving specific molecular detection
光纤化学传感器还具有表面分子识别位点或化学反应部位，可用于特定分子的检测。 - The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27 . it was released from the cbd fusion tag efficiently
利用融合蛋白中目的蛋白lt 27与cbdtag接头处的肠激酶特异性识别位点，用肠激酶处理粗纯化的融合蛋白，可将卜 - A novel calix [ 4 ] arene - based fluorescent chemosensor bearing 2 - aminopyridine moiety and naphthalenic fluorphore was synthesized , and the chemical structure of the product was identified by ft - ir , ms - fab , nmr and elemental analyses
摘要以杯[ 4 ]芳烃为平台、氨基吡啶基团为识别位点、蔡环为荧光基团，合成了一类新型荧光化学传感器。 - After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。 - Total rna was extracted from hepatic cells of mouse . a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r , and endostatin was amplified by rt - pcr , this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively
从小鼠肝脏细胞中提取总rna 。设计合成一对特异引物，分别带有ecori和bamhi的限制性内切酶的识别位点。用rt - pcr法扩增endostatin的基因片段，在endostatin基因的两侧引入ecori和bamhi酶切位点。