裂合 meaning in English
lyase
Examples
- We report here the cloning of a homolog of this gene from dunaliella salina . this cloned gene produces as protein with photolyase activity when expressd in escherichia coli
运用blast方法对est序列进行分析，初步预测该est序列为光裂合酶蓝光受体蛋白质家族中一个成员的部分cdna 。 - 5 . after induced with iptg , jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds , cultivate these e . coli with light or without light . the survival rate proved the gene of d . salina ( 6 - 4 ) photolyase has photoreactivation
5 .工ptg诱导过的转化菌，经过30秒的紫外光照射后，分别进行光培养或暗培养，计算存活率，从而证明了及sa了z ’ na的( 6一4 )光裂合酶在大肠杆菌中可进行光修复活性作用。 - It " s the first cdna code ( 6 - 4 ) photolyase found in low orgnism ( alga ) , and this study is important for the reseach of 6 - 4 photolyase . the methods and analysis as bellows : 1 . this est was analyzed by method of blast , and the conclusion suggests that the sequence probably be a partial cdna that can code one of protein family of photolyase / blue light photoreceptor
利用3 ’ race法扩增此cdna序列的3 ’端部分，所得片段长度为2575bp ，含一个完整的开放读框，长1800bp ，可编码600个氨基酸；利用生物信息学法对此扩增序列进行分析，包括同源性分析、序列的读框分析、蛋白质的保守性分析以及该蛋白的进化关系分析，预测出此序列编码了d . salina的( 6 - 4 )光裂合酶。 - Many organisms produce enzymes , termed photolyases , that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ) , thereby reversing the damage . these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ) . a gene that expresses a protein with 6 - 4 photolyase activity in vitro , was recently cloned from high organisms ( arabidopsis thaliam , drosophila melanogaster , danio rerio , xenopus laevis and homo sapiens )
目前已从高等生物拟南芥、鲐类、果蝇、人类和非洲爪蟾蜍属中克隆到有( 6 - 4 )光裂合酶活性的基因，本研究从盐生杜氏藻dunaliellasalina中克隆到( 6 - 4 )光裂合酶的基因，并将该基因在大肠杆菌中得以表达，这是首次在藻类中克隆到( 6 - 4 )光裂合酶基因，对光裂合酶的研究具有重要意义。