表达克隆 meaning in English
cloning by expression
Examples
- Of the differentially expressed clones , 104 clones were highly expressed in the ovary at stage iii , the remaining clones were highly expressed in the ovary at stage ii
结果共获得2倍以上差异表达克隆167个。其中期高表达克隆104个,期高表达克隆63个。 - Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible
结论成功构建了echistatin的原核高效表达克隆,表达量高于现有国内外研究水平,为echistatin功能及相关疾病的研究奠定了基础。 - Chapter 3 sequencing cdna fragments of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) partial clones were sequenced which were selected from the differentially expressed clones screened by cdna macroarray analysis
3中华绒螯蟹卵巢发育相关基因cdna片段的序列测定从cdna微阵列筛选的差异表达克隆中选取部分克隆进行测序。 - Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully . 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr . after sds - page and densitometric scan analysis , the expression level of r hpf4 is 25 - 30 %
结论本研究运用pcr定点突变技术,完全去除了hpf4cdna基因3 ”端utrat富含区:改用大肠杆菌强串联终止密码子taaaataa ,成功构建高效表达克隆pbv220 rhpp4 。