表观分子量 meaning in English
apparent molecular weight
Examples
- Cloning and expression in pichia pastoris of an alkaline mannanase gene
1基因重组胞外菊粉酶表观分子量为90 - The apparent molecular weight of g6pd , determined by gel filtration , was 220kd ; the subunit molecular weight was 50 . 5kd as determined by sds - page , suggesting that the native enzyme was a tetramer consisting of four identical subunits
凝胶过滤法测定该酶表观分子量约为220kd ， sds - page测定亚基分子量约为50 . 5kd ，可见该酶是由四个相同亚基构成的四聚体。 - The purified lectin showed a single protein band on sds - page , and the subunit molecular weight was 12kd . the molecular weight was 45kd determined by gel filtration on sephacryl s - 100 . the result implied that there are four same subunits per lra
经sds - page检测为单一蛋白带，亚基分子量为12kd ， sephacryls - 100凝胶过滤测得其表观分子量为45kd ，表明lra是由四个相同亚基组成的蛋白。 - Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction , fraction with ( nhi ) 2864 , hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr . cml agglutinated type a human red blood cells specially . the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis
野花生豆( crotalariamucronata )经磨粉、浸取、硫酸铵分级沉淀、猪胃粘蛋白- sepharose4b亲和层析、 sephacryls - 200hr分子筛层析可得到一表观分子量为103kd且对a型血红细胞专一凝集的野花生豆凝集素( crotalariamucronatalectin ， cml ) 。 - Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies
重组质粒转化巴氏毕赤酵母， g418筛选出多拷贝插入的单克隆，甲醇诱导多拷贝插入的单克隆酵母细胞分泌目的蛋白，培养液上清经sds - page电泳分析，在蛋白质印迹中检测到培养液上清有一表观分子量为36kd ，能与羊抗hcmv多克隆抗体发生发应的条带。