蟋蟀总科 meaning in English
grylloidea
Examples
- 2 ) the method of proteinase k isolation and the method of ctab isolation had been compared carefully while extracting the genome dna from crickets . the result showed the method of proteinase k isolation of total dna was more suitable for the extraction of total dna from insects of grylloidea
2 ）在提取蟋蟀总科昆虫的基因组dna时，比较了ctab法和蛋白酶k法两种方法，结果显示蛋白酶法更适用于蟋蟀总科昆虫的基因组则a的提取。 - Among 54 arbitrary primers tested , only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae
本研究经用54种随机引物对57种蟋蟀的rapd扩增分析得出以下结论： 1 ）采用rapd技术，首次较系统地对中国蟋蟀总科7科26属共57种蟋蟀进行了系统学研究。在所试用的54种随机引物中筛选出可扩增出清晰可辨且稳定的rapd图谱的引物分别为：蟋蟀科9种、岭蟋科9种、树蟋科12种、结蟋科12种、蛛蟋科13种、铁蟋科13种、貌蟋科13种。 - In the present paper , some investigations on loxoblemmus doenitzi ( gryllidae ) are reported . in order to discover the meiosis of spermatomeres and spermiogenesis , slice - pressing method and improved phenol - fuchsin dyeing method are used . in the course of meiosis , primary spermatocytes directly enter metaphase ii , not through telophase i and prophase ii
本论文以直翅目、螽斯亚目、蟋蟀总科、蟋蟀科的多伊棺头蟋( loxoblemmusdoenitzi )为材料，采用压片法-改良苯酚品红染色，研究了精子发生过程中的减数分裂阶段和精子形成，发现初级精母细胞第一次减数分裂后不经过末期和前期直接进入中期。 - 2 . 5 ul of 10 x reaction buffer , 1 . 5 ul 25mm mgcl2 , 0 . 3 ul lomm dntp , 0 . 5 ul taqdna polymerase ( 5 u / ul ) , and lul ( = 20ng ) of primer were used in per reaction . each reaction was overlaid with one drop of paraffin oil . the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec , annealed at 37 1 min , extended at iv b 72 for 2 min and repeated the cycle 45 times , at last , extended at 72 ' c for lomin
等( 1995 )利用rapd标记区分美国东部一杂交地带的蟋蟀的两个姐妹种， allonemobiusfasciatus和a . socius ，并于1998年使用rapd和异型酶标ic做出了这两种蟋蟀的基因连锁图；国内田英芳、郑哲民（ 20of ）首次将rapd技术运用于蟋蟀总科的分子系统学研究中，采用2种引物对7种蟋蟀进行了基困组dna多态性研究，并应用upg问a法构建树状图，椎测系统发生关系 - Thermocycled pcr samples were resolved electrophoretically on 1 . 5 % agarose gels and taken photos using a polaroid camera . the statistical results were analyzed by the spss software , and the cluster figures were obtained . conclusions could be drawn from the study as following : 1 ) the molecular systematics of 57 species of crickets , which belong to 26 genera 7 family in grylloidea , had been studied by the approach of rapd
本项研究在依据外部形态分类鉴定及前人工作的基础上，采用rapd技术，通过对蟋蟀总科7科26属57种蟋蟀基因组dna的rapd图谱的比较研究，在分子水平上探讨这些类群的分类地位和亲缘关系，为丰富蟋蟀总科的分子系统学研究，并为进一步完善蟋蟀总科的分类系统，揭示其系统发育及演化提供分子水平的依据。