蛋白质印迹 meaning in English
western blot
Examples
- 3 ) western blotting of gas1p when mutant gpi17p overexpressed showed that there was accumulation of immature gas1p . this means that there is a defect in gpi - anchoing
3 ）蛋白质印迹分析表明，在突变gpi17p存在下，有未成熟gas1p的积累，表明存在蛋白质阶i化缺陷。 - Western blot experiments indicated that the partially purified antibody preparations reacted specifically with the pks proteins expressed in e . coli and , as expected , also with the very large pks proteins naturally present in s . sp
蛋白质印迹实验显示，部分提纯的抗体样品与大肠杆菌中表达的pks蛋白有特异性反应。同时，这些抗体也可与天然存在于链霉菌fr - 008的、巨大的pks蛋白特异性地反应。 - The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified . sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e . coli
从转化的平板中筛选出阳性重组子，进行不同iptg浓度和不同诱导时间的表达研究，并利用sds - page电泳和westernblotting蛋白质印迹技术对外源基因cei _ ( 12 )在大肠杆菌e - Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies
重组质粒转化巴氏毕赤酵母， g418筛选出多拷贝插入的单克隆，甲醇诱导多拷贝插入的单克隆酵母细胞分泌目的蛋白，培养液上清经sds - page电泳分析，在蛋白质印迹中检测到培养液上清有一表观分子量为36kd ，能与羊抗hcmv多克隆抗体发生发应的条带。