营养缺陷型 meaning in English
auxotrophy
Examples
- Tatum isolated a number of different auxotrophs of e. coli.
Tatum从大肠杆菌中也分离出若干种不同的营养缺陷型。 - Tatum isolated a number of different auxotrophs of e . coli
Tatum从大肠杆菌中也分离出若干种不同的营养缺陷型。 - Tatum isolated a number of different auxotrophs of e . coli
Tatum从大肠杆菌中也分离出若干种不同的营养缺陷型。 - In this article , the advanced structure of hybrid peptide mae is predicted with software . the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2 , and then it is cloned into expression vector plasmid ppic9k . after verified by restriction enzyme analyzing and sequencing , the vector is transferred into the eukaryotic host ( yeast pichia
并以已构建的载体ptyb2 - mae为模板，通过pcr扩增出融合基因mae - imein - cbd ，将其克隆于表达质粒ppic9k中，通过鉴定并测序正确后，电转化真核表达宿主? ?毕赤酵母菌株gs115 ，通过营养缺陷型培养基筛选重组子，再利用g418抗性筛选出整合有多拷贝外源基因的重组子。 - In this article , the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression . adopted a new strategy , multiple copy gene is ligated in the same direction . and then it is cloned into expression vector plasmid ppic9k
本文根据genebank登录的氨基酸序列，同时考虑构建和表达的需要，化学合成了misgurin基因和接头，采用一种新的策略，在体外将基因多拷贝同向串连，并将其克隆于表达质粒ppic9k中，通过鉴定并测序正确后，电转化真核表达宿主? ?毕赤酵母菌株gs115 ，通过营养缺陷型培养基筛选重组子，再利用g418抗性筛选出整合有多拷贝外源基因的重组子。