荧光淬灭 meaning in English
quenching of fluorescence
Examples
- The fluorescence quenching of polygonatum odoratum mill . druce lectin
凝集素的荧光淬灭研究 - Inorganic carbon transport was a major factor in facilitating quenching during the initial stage and the initial rate of fluorescence quenching in the presence of iodoacetamide , an inhibitor of co2 fixation , was 87 . 9 % of control
初始阶段的荧光淬灭主要是由于无机碳转运所致，加入co _ 2固定的抑制剂iodoacetamide ，荧光淬灭的初始速率为对照的87 . 9 。 - . from the direct mutant of spirulina platensis ( sp - d ) , we got high purity and activity phycobiliprotein which could grow crystals . the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表达的peac1为抗原制备了免疫活性较好的抗豌豆肌动蛋白的多克隆抗体，从螺旋藻中纯化了高纯度、高活性、能结晶的藻胆蛋白，将两者偶联制备的藻荧光探针，不仅保持了藻胆蛋白很强的抗荧光淬灭能力，而且用于豌豆卷须气孔细胞荧光标记时有更低的荧光背景。 - I used acrylamide ( acr ) and ki to probe the micro - environments of trp residues in the g6pd molecule to show that the trp residues fluorescence of g6pd were quenched by the two quenching reagents , 100 % and 78 % was quenched by acr and ki respectively . i deduced that some trp residues of g6pd were located in the inner core and the others located in the polarity surface
用常见的荧光淬灭剂丙烯酰胺和ki研究g6pd分子中trp残基的微环境，发现它们对分子中t印残基的荧光均有淬灭作用，丙烯酸胺能淬灭100 %的荧光， kl能淬灭78 %的荧光，表明分子中小部分饰残基埋藏在分子内部，大部分t印残基处于分子表面的极性环境中。 - Ag + and fe2 + caused the fluorescence of trp residues in g6pd quenched ; mg2 + and edta made fluorescence intensity of g6pd increased , this indicates that they caused trp residues wrapped and came to the inner core and located in the hydrophobic area ; while zn2 + or mn2 + made fluorescence intensity of g6pd decreased , this indicates that they made the conformation of g6pd relaxed and chromophores exposed to polarity environments . in native condition and in the far circular dichroic ( cd ) region , g6pd exhibited two characteristic negative band centered at 208nm and 222nm respectively , thus it is estimated to contain about 41 . 2 % a - helix , 20 . 6 % - pleated sheet and 38 . 2 % random coil and turn
Ag ~ +和fe ~ ( 2 + )引起色氨酸( trp )残基的荧光淬灭； mg ~ ( 2 + )和edta均使g6pd的荧光强度增强，说明它们使trp残基重新包裹在分子内部而处于疏水的微环境中； zn ~ ( 2 + )和mn ~ ( 2 + )均使g6pd的荧光强度变小，说明它们使酶分子构象变得疏松，原来处在分子内部的发色团暴露在极性环境中。