荧光染色法 meaning in English
fluorescent staining method
Examples
- The survival - rate of the recovered cells was tested by mtt and fda - pi . therefore , we got the preferable condition of vitrification , i . e . , the concentration of cryoprotectant is 60 % and the time of disposal is 15min . under this condition , the value of fda / pi is 46 . 43
冻融后的细胞经过mtt检测和fda - pi双荧光染色法检测，获得预处理过渡的优化条件，即60浓度的保护剂，预处理15分钟，此时的fda pi值为46 . 43 。 - Materials and methods the mouse , golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation , respectively , and ar was induced by progesterone after capacitation , then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method . the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf , then the fertilization rate was observed . the 1 - cell , 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin , then the rate of blastocysts was recorded
方法取小鼠精子10份、金黄地鼠精子6份、人新鲜精液标本10份及人冷冻精液标本9份，分别与不同浓度内毒素共孵育进行体外获能和孕酮诱导的顶体反应，应用金霉素和dna结合的荧光染料hoechest33258双重荧光染色法检测精子的获能率和顶体反应率；小鼠体外受精实验的精卵结合环节培养液中加入不同浓度的内毒素，观察受精情况并记录受精率；取小鼠1 -细胞胚胎、 2 -细胞胚胎和去卵透明带2 -细胞胚胎，与不同浓度内毒素共孵育进行体外培养，观察体外发育情况并记录囊胚率。