色氨酸酶 meaning in English
tryptophanase
Examples
- Addition of iptg to growing culture of the lysogen induces t7 rna polymerase , which in turn transcribe the target dna in the plasmid . in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg , a 52kd protein with tryptopanase activity was expressed
摸索发酵条件，如改变培养温度和iptg浓度等，发现在30培养条件下， 0 . 2mmiptg诱导时，发酵液中的吲哚含量最高，表明低浓度的诱导剂或低温诱导有利于表达出有活性的色氨酸酶。 - Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase . the fermentation conditions were optimized respectively . in the presence of glucose and iptg as induce agent , the concentration is the crucial factor for expression of active tryptophanase . if the iptg concentration is less than 0 . 2mm , the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg
用iptg诱导表达实验结果表明：利用t7启动子在胞内能表达出有活性的酶； iptg与温度的合适组合可以得到较高活性的色氨酸酶，在0 . 2mm以下浓度的iptg诱导时， 37是最适温度，而用较高浓度的iptg诱导，低温是表达有活性的色氨酸酶的必要条件，而且诱导时间要短。 - Using pcr technology , a 2 . 4kb dna fragment , part of tryptopanase operon , containing a promoter , a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e . coli k - 12 , was cloned to pmd18 - t vector . the dna sequence is the same as which was published on science
为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个dna片段克隆到pet28c质粒的bamhi和hind位点上，使该片段受t7rna聚合酶的启动子控制，然后转化噬菌体de3的溶源菌bl21 ( de3 ) 。