致死作用 meaning in English
lethal effect
Examples
- The transformation frequency achieved lo ^ cells / ug dna when pseudom onas fluorescent , was grown to 0 . 55 at odr , oo , and incubated for 2 minutes at 42 xh prior to treatment with 25mmol / l cacl2
获得克隆子lzng -一20a和lzr19一20a .在iptg诱导下，不管插人的尸2叹基因是正向还是反向，都能保护cyt人蛋白对大肠杆菌的致死作用 - Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae , extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates . the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents . a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用线虫诱导下owvt - 1菌株液体发酵，通过粗分级分离、离子交换层析和凝胶过滤层析分离提纯了一个分子量为31kda的丝氨酸蛋白酶，生物学测定表明其对大豆胞囊线虫二龄幼虫具有致死作用，同时测定了该酶理化特性，酶活力在75附近酶活力最高，随着ph的增加酶的稳定性升高，与胆碱酯酶具有相似的ph曲线，对特异性底物aape ( suc - ala - ala - pro - glu - pna )具有作用， ssi和ci - 2抑制该酶的活性。