胰酶消化 meaning in English
pancreatic digestion
trypsinization
trypsinize
Examples
- The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
应用胰酶消化法进行颌下腺细胞的分离、纯化及原代培养、传代培养。 - Methods : low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture
方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。 - Cells were centrifuged for 5min at 1000rpm and removed supernatant . the cell peller was resuspended in 1 ml culture media and divided into two glass flask . cell surface makers ( cd45 , cd90 ) were detected by immunocy - tochemistry
当传到第五代时,取一瓶mscs用胰酶消化,以8x10vcm ‘浓度接种在六孔板中的盖玻片上,应用免疫细胞化学方法检测cd45人d90的表达。 - To study the suitable method for cattle oviduct simple epithelium cells culture , the epithelium cells were isolated by cutting and 0 . 25 % trypsinization , the exponential phase of growth cells vigor and growth velocity was determined by mtt method , the viable count was detected by the rejection experiment of trypanblau
摘要为探讨适用于黄牛输卵管单层上皮细胞的培养方法,采用机械剪取及0 . 25 %胰酶消化的方法分离获得上皮细胞,取对数生长期细胞进行mtt比色实验检测细胞活力和生长速度;台盼蓝排斥试验检测活细胞数。