胍法 meaning in English
guanidine method
Examples
- In the experiment , the first instar housefly larvae " metallothionein mrna expressing reach maximur . after induced by cd ( 2mm ) for 48 hours , then total rna was extracted with guanidinium isothiocyanate , and mrna was isolated by the polyattract mrna isolation systems surplied by promega company
试验中采用2mmcd ~ 2对家蝇初孵幼虫诱导48小时，使其体内金属硫蛋白mrna表达达到高峰，在液氮中将其研磨成粉末后采用异硫氰酸胍法提取总rna ，再用promega公司提供的小量分离mrna试剂盒纯化mrna 。 - The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method , then the total rna digested by dnase that had not rnase was used for rt - pcr . i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition . at the end i selected the magnesium ion density as 1 . 25 mm . the production of rt - pcr was inserted directionally into pgem ? z ( ampr ) . the pgem ? z ( ampr ) was used to transform e coli jm109 . i got a positive clone through culling and identificatin . the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取诱导培养液中的菌体，用异硫氰酸胍法提取总rna ，总rna再经无rna酶的dna酶处理后用于rt ? pcr 。在pcr扩增目的基因时，通过优选扩增体系，使镁离子浓度为1 . 25mm时rt ? pcr可顺利地获得目的基因，并能定向克隆到载体pgem ? 3z ( amp ~ r )中。用克隆载体转化宿主大肠杆菌jm109 ，通过筛选获取阳性克隆子，对阳性克隆子进行酶切与pcr鉴定，并对载体中插入的目的基因进行测序。