肿瘤细胞系 meaning in English
tumor cell lines
Examples
- Curcumin has been shown to induce a wide variety of tumor cells by several mechanisms , mainly including modulation of the expression of oncogenes and cancer suppressor genes , down - regulation the activation of transcription factors , via many signal transduction pathways , and the modulation of cell cycle . provides an overview of domestic and international apoptosis mechanisms of malign tumor cells induced by curcumin in order to better explore and open up new avenues of cancer treatment
姜黄素能够诱导多种肿瘤细胞系凋亡,其机制主要是调控癌基因和抑癌基因,下调多个转录因子活性,通过多种信号转导途径,诱发细胞周期停滞而诱导细胞凋亡.对近年来姜黄素诱导细胞凋亡的机制进行综述,以便更好地探求和开辟治疗恶性肿瘤的新途径。 - Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 - Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry . the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines , and compared with pgp inhibitor quinidine . the pgp expression of mcf - 7 adr was strongly positive , the positive rate was 15 % ; the pgp expression of mcf - 7 was negative , the positive rate was 1 . 8 % . ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115 . 2g ml and 207 . 6g ml respectively . ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129 . 8mol l 42 . 1g ml and 94 . 1mol l 30 . 5g ml respectively . tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7 , but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly . immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively . tea polyphenol is not only an anti - tumor agent , but also a multidrug resistant modulator similar as quinidine . tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫组化法和流式细胞仪对肿瘤细胞系mcf - 7和mcf - 7 adr的p -糖蛋白表达水平进行定性定量研究。用噻唑蓝比色法mtt研究茶多酚的细胞毒性及其对耐药性的逆转作用，并与pgp抑制剂奎尼定进行了比较。免疫组化法检测p -糖蛋白表达水平， mcf - 7 adr呈强阳性，而mcf - 7呈阴性流式细胞仪定量检测结果mcf - 7 adr细胞系细胞阳性率为15 % ， mcf - 7细胞系细胞阳性率为1 . 8 % 。