肽质指纹图谱 meaning in English
peptide mass fingerprinting
Examples
- In he - 3 and de - 3 , 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously . in he - 2 and de - 2 , 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously , some of these different proteins are correlative with eyes development , nerve development , development regulation , cell differentiation and maintenance , arthromere formation and signal transduction . it shown that gene expression have significant difference between diploid and haploid during embryos developing
分别选取其中70个分辨好的差异蛋白质点进行原位胰蛋白酶酶解，酶解的肽片段用基质辅助激光解析电离分行时间质谱( maldi - tofms )得到肽质指纹图谱，再在网上peptident软件中搜索swiss - prot和trembl数据库，初步鉴定了he - 3和de - 3上32个差异蛋白质点，其中包括16个表达量上有差异的蛋白质点( p 0 . 05 )和16个有无表达的差异蛋白质点，并且初步鉴定了he - 2和de - 2上25个差异蛋白质点，其中包括5个表达量上有差异的蛋白质点( p 0 . 05 )和20个有无表达的差异蛋白质点。 - For protein identification , proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised , digested in - gel with trypsin , and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ) . erp60 , trypsinogen , proelastase , lipaseandso on were identified . this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development
从中随机选择一些差异蛋白质点，进行基质辅助激光解吸-电离飞行时间质谱( maldi - tof - ms )测定其胶内酶解后的肽质指纹图谱，用mascot软件查询swiss - port数据库，初步鉴定为一些与生长发育、物质代谢、细胞因子、信号转导等有关的蛋白质，如erp60 、 trypsinogen 、 proelastase 、 lipase等。