肝细胞培养 meaning in English
liver cell culture
Examples
- Liver cell culture
肝细胞培养 - The number of adherent cells in special medium was more than that in common medium , but the morphologic difference between two groups was not significant at that time . the difference became significant after 24 hr of culture
肝细胞培养液组贴壁的肝细胞数目明显多于普通培养液组，但此时两组培养液中的小鼠肝细胞在形态学上的差异并不显著。 - These cells were polygon and displayed morphologic characteristics of liver parenchymal cells at 24 hr of culture , such as a large round nucleus with a few nucleoli and many cytoplasmic granules , sometimes binucleate , hepatocyte was in direct contact with adjacent cells
而肝细胞培养液组在低倍镜下观察，培养瓶的背景干狰，死细胞较少，贴壁细胞伸出多个伪足，呈多边形。相邻细胞连接成小片状。 - Methods : hepatocytes obtained from mice with in situ portal vein collagenase perfusion were inoculated on two types of medium , which one was 10 % pcs rpmi 1640 medium ( common medium ) , the other was rpmi 1640 medium supplemented with 5 u g / ml tranferrin , 5 u g / ml insulin , 10nm nicotinamide , 5mm - me , 40 u g / ml hgf ( special medium )
一种是在rpmi1640培养液中加入了511iml转铁蛋白、 5pg ml牛胰岛素、 10nm烟酚胺、 smm卜琉基乙醇， 4011g ml促肝细胞生长因子，简称为肝细胞培养液。