终浓度 meaning in English
final concentration
Examples
- Expression was induced by adding iptg to a final concentration of imm and incubation with shaking at 30 for 3 h
以2 %比例接种入新鲜mg培养基,培养至对数中期,加入iptg至终浓度为1mm ,在温度为30的条件下诱导表达3小时。 - The different bivalent cations with the same final concentration were put into the dnaase reacting system for 15 minute at ph5 . 4 , at 41 , then dnaase ' s activity unit was detected
在dna酶的酶促反应体系中加入终浓度相同的二价金属阳离子41下、 phs 4准确反应15分钟后测定酶的活力单位。 - When ultima concentration of iptg was 1 . 0mmol / l and induced for 3 hours , the protein expression quantity was the most candidate : zhao chunli major : basic veterinary science supervisor : prof . haoyanhong prof . liqingzhang
对表达蛋白的定量分析得出,当iptg终浓度为1 . 0mmol l ,诱导3个小时蛋白表达量最大。 - Cl ; 100mm nacl ; 0 . 5mmol / l edta ; 4mol / l carbamide ; 0 . 2 % ( v / v ) tritonx - 100 ; 0 . 2 % doc ) , we got r hpf4 with the purity of 85 % . the yeild is 120mg recombinant protein per litre culture
Rhpf4纯度可达85以上。 rhpf4e透析的同时,采川立气氧化法,加以cu ‘至终浓度川” mol 1加速空气氧化,使rhpf4上确显折叠。 - When the odsoo of the bacterial solution is 0 . 5 , we add iptg to the control tubes and make their terminal concentration to immol / l . then continue to culture them for another 2 hours . collect the inducing and non - inducing e . coli dh5a by centrifuge and sds - page
5时,于诱导对照管中加入iptg至终浓度为inunoffe :继续培养二小时,将离心收集的诱导及未诱导的细菌沉淀进行sds page 。