细胞冷冻 meaning in English
cell freezing
Examples
- Players are freezing the cells taken from the umbilical cord blood of their babies as a possible future cure for cartilage and ligament problems
球员将孩子脐带血中的干细胞冷冻，以便今后可以用于治愈软骨和韧带疾病。 - The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以细胞培养液m199 (含2既的小牛血清，常规量双抗)为冻存稀释液对泥鳅胚胎细胞冷冻保存宜采取先慢后快的方式(例如，从0一一6 ，冻存速度为一0 . 05 / min ，再以一0 . 5 / min的速度从一6一一40 ) ; dmso的保护效应浓度为12 . 506左右;小牛血清的浓度对泥鳅胚胎细胞的成活率影响不明显;囊胚晚期细胞抗冻性比中早期强;通过对不同批次的冻存细胞解冻培养，解冻后成活率为30 %以上细胞培养数天后均有少数细胞贴壁，但只发现两瓶培养细胞有明显增殖现象产生许多未分化的小细胞。 - Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus , two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196 . after 6 days cryopreservation , one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other , cryopreserved for 13 days , was 52
以细胞培养液m199 (含20 %的小牛血清)为稀释液， dmso的浓度为11 % ;与泥鳅胚胎细胞冷冻保存方法一样，采取先慢后快的方式，冷冻保存两组草鱼囊胚晚期细胞于一1 %的液氮中。第一组冷冻保存6天后解冻，成活率为72 % ，第二组冷冻保存13天后解冻，成活率为520 / 0 。 - Different from mammals , the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ) . therefore , three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition . researchers have had systematically studied on this technique for many years , and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc . ) , and through the process of culturing histiocyte of fish , cryopreservation and re - culture after thawing , carrying out somatic cell breeding of fish . the past studies showed that the nucleolus of somatic cells of fish have totipotency
多年来，国内外学者对各种鱼类精液的冷冻保存进行了大量的系统研究，目前这项技术已达到实用水平，并日益发挥作用；二是对鱼类培养的组织细胞冷冻保存，通过鱼类细胞的培养、超低温冻存、解冻后再培养过程，结合细胞工程技术(如核移植、电融合等)进行体细胞育种；大量的研究结果表明鱼类体细胞核具有发育的全能性，随着细胞培养技术、细胞工程技术日益发展成熟，完全具备实现鱼类物种种质长期保存的理论基础和技术条件。